Abstract
A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving ~10 nm z position resolution by parameterising the images with an image sharpness metric. © 2010 Optical Society of America.
| Original language | English |
|---|---|
| Pages (from-to) | 877-884 |
| Number of pages | 8 |
| Journal | Optics Express |
| Volume | 18 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 18 Jan 2010 |
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Dalgarno, P. A., Dalgarno (nee Campbell), H., Putoud, A., Lambert, R., Paterson, L., Logan, D. C., Towers, D. P., Warburton, R. J., & Greenaway, A. H. (2010). Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy. Optics Express, 18(2), 877-884. https://doi.org/10.1364/OE.18.000877
Dalgarno, Paul A. ; Dalgarno (nee Campbell), Heather ; Putoud, Aurelie et al. / Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy. In: Optics Express. 2010 ; Vol. 18, No. 2. pp. 877-884.
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title = "Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy",
abstract = "A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving ~10 nm z position resolution by parameterising the images with an image sharpness metric. {\textcopyright} 2010 Optical Society of America.",
author = "Dalgarno, {Paul A.} and {Dalgarno (nee Campbell)}, Heather and Aurelie Putoud and Robert Lambert and Lynn Paterson and Logan, {David C.} and Towers, {David P.} and Warburton, {Richard J.} and Greenaway, {Alan H.}",
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Dalgarno, PA, Dalgarno (nee Campbell), H, Putoud, A, Lambert, R, Paterson, L, Logan, DC, Towers, DP, Warburton, RJ & Greenaway, AH 2010, 'Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy', Optics Express, vol. 18, no. 2, pp. 877-884. https://doi.org/10.1364/OE.18.000877
Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy. / Dalgarno, Paul A.; Dalgarno (nee Campbell), Heather; Putoud, Aurelie et al.
In: Optics Express, Vol. 18, No. 2, 18.01.2010, p. 877-884.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy
AU - Dalgarno, Paul A.
AU - Dalgarno (nee Campbell), Heather
AU - Putoud, Aurelie
AU - Lambert, Robert
AU - Paterson, Lynn
AU - Logan, David C.
AU - Towers, David P.
AU - Warburton, Richard J.
AU - Greenaway, Alan H.
PY - 2010/1/18
Y1 - 2010/1/18
N2 - A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving ~10 nm z position resolution by parameterising the images with an image sharpness metric. © 2010 Optical Society of America.
AB - A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving ~10 nm z position resolution by parameterising the images with an image sharpness metric. © 2010 Optical Society of America.
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Dalgarno PA, Dalgarno (nee Campbell) H, Putoud A, Lambert R, Paterson L, Logan DC et al. Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy. Optics Express. 2010 Jan 18;18(2):877-884. doi: 10.1364/OE.18.000877
